Based on the summary of the domestic and international development status of clinical pathway and the disadvantages of paper-based clinical pathway management，the paper proposes measures for constructing ontology-based electronic clinical pathway conceptual framework. With the basis of "Breast Cancer Clinical Pathway (2009 Edition)" promulgated by the Ministry of Health, with the combination of medical subject thesaurus, specialized textbooks and related guidance on breast cancer treatment, the paper also conducts an empirical study on constructing breast cancer clinical pathway ontology conceptual framework, which provides a theoretical reference to the clinical pathway electronic management system construction, it is helpful for medical information resources development and utilization.