Taking students from Anhui Medical University as target group, the effect of evidence-based medicine course to information literacy training is investigated. Intervene group includes the students who take medical literature retrieval course and evidence-based medicine course at the same time, the control group includes the students who take medical literature retrieval course only, it carries out investigation from the aspects of information technology application, information access, information evaluation capability. The results reveals that the information literacy of intervene group is better than the control group, the combination of evidence-based medicine with literature retrieval course is helpful for information literacy education.