The paper elaborates on the design and implementation of the wisdom laboratory management information system for Zhengzhou Center for Disease Control and Prevention (CDC). It introduces the system architecture, database environment, construction principles, characteristics, functions and user management. Featured by its high sharing ability, security and transportability, now it has been applied successfully in Zhengzhou CDC and its subordinate county- and district-level CDCs and realizes the big data analysis, management and decision-making based on information and intelligence.