Through literature method, expert consultation method, K-means algorithm and empirical analysis method, the paper analyzes the methods for evaluation on the layout of first-aid sites, discusses the indexes for scientific evaluation on the rationality of layout of first-aid sites, conducts visualized and practical calculation based on the first-aid related data of Wuhan city in 2015, verifies the rationality and operability of the evaluation indexes, and provides reference for overall allocation of first-aid resources and rational layout of first-aid sites.