Based on the problems existing in the original specimen management process of the First Affiliated Hospital of Guangdong College of Pharmacy, the paper proposes achieving the closed-loop management process of laboratory specimens through wireless network technology, PDA, laboratory specimen bar code, QR codes of patients and interaction of multiple systems, and describes the functional design and application effect of the system. The application of the closed-loop management process for laboratory specimens can better standardize and normalize the management of specimens.