The paper issues questionnaires to investigate the current status of informatization construction and application in 32 provincial disease prevention and control institutions of China, summarizes the current status of informatization organization and management, standard planning, capital input and allocation, analyzes the problems existing in the process of disease control informatization construction, focuses on the discussion of imbalanced regional development, insufficient capital input, lack of informatization personnel, and lack of informatization security awareness, and proposes corresponding suggestions and countermeasures.