Taking 25 medical university libraries as the survey objects, the investigation and analysis is conducted from three aspects of the organizational mode, service form, and service content of subject service, summarizes the service status and existing problems, proposes service strategies for medical subject, including "university + hospital" medical library alliance, "teaching + scientific research + clinical + library" subject service team, embedded "medical education scientific research" subject service form, etc., expounds on the subject service content based on medical characteristics.