A cluster analysis is carried out on the high-frequency subject terms appearing in the literatures related to Artificial Intelligence(AI) in biomedical field in recent three years included in PubMed database. According to the clustering results, the paper analyzes the current study hotspots in this field, including five topics of clinical medicine and bioinformation, namely, computer-aided diagnosis of diseases, classification of brain states for stroke rehabilitation and detection of Alzheimer's disease, biometrics, biological nervous system study.