Based on the thematic framework analysis method, the paper compares and analyzes the building situation of public health informatization projects of four typical cases of Minhang District of Shanghai City, Xiamen City of Fujian Province, Yichang City of Hubei Province, Yinzhou District of Ningbo City from the aspects of the building process, operation mechanism, implementation effect, etc., summarizes the experience and enlightenment, and provides references for other regions to further promote the building of public health informatization.