Multi-source heterogeneous resources such as encyclopaedia, clinical guidelines, scientific literature and expert experience are used to define the top-level category of ontology, and ontology tools such as Ontofox and Protégé are used to collect and organize top-level concepts and terms of melanoma diseases, molecular abnormalities, symptoms, anatomical structures, drugs, interventions and procedures, complications, etc. The single-disease ontology of melanoma is constructed and evaluated, the ontology visualization and retrieval tools are developed, and some useful references are provided for ontology construction research.