The paper introduces the framework of the training mode for innovative applied talents for the internet of things(IoT) engineering major based on the integration of medical and information technology, school-enterprise collaboration, expounds the technical route and implementation approach of the teaching practice under this mode, including the establishment of school-enterprise collaborative multi-level practical teaching system, the construction of four-level curriculum system of multi-discipline integration, the establishment of multi-dimensional learning community and the establishment of three-fold guarantee mechanism, and analyzes the effect of promotion and application.