Purpose/Significance Geographic information system (GIS) technology is used to preliminarily explore and analyze the spatial aggregation and visualization processing of Nanjing tertiary general hospitals and to provide references for rational allocation of medical and health resources. Method/Process Tyson polygon,average nearest neighbor analysis and Ripley’s K function are used to describe and analyze the spatial distribution characteristics of tertiary general hospitals in Nanjing.Result/Conclusion The maximum area of the Tyson polygon is 285 times the minimum area. The nearest neighbor ratio is 1.009 7,Z=0.092 3,P=0.926 4. When weighted with the reciprocal of population densities of districts,the observed K value is significantly lower than the expected K value.When only the spatial location is considered,the distribution of tertiary general hospitals in Nanjing tend to be randomly distributed; While weighted with the reciprocal of population densities,the distribution becomes discrete.