Purpose/Significance The paper studies the application scenarios and typical cases of artificial intelligence (AI) in libraries of medical universities and colleges, analyzes the main stakeholders and their interest demands, and puts forward suggestions for application and promotion. Method/Process Literature research and telephone consultation are used to determine the application scenarios and typical cases of AI in libraries of medical universities and colleges, and the Mitchell scoring method and Clarkson classification are used to identify major stakeholders, and the interests and problems faced are analyzed. Result/Conclusion The application scenarios of AI in libraries of medical universities and colleges mainly include three dimensions:environment, resources and services. The main stakeholders include governments, medical universities and colleges, libraries of medical universities and colleges, librarians, readers and AI vendors. In view of the existing problems, countermeasures and suggestions are put forward from four aspects.