Purpose/Significance To explore the establishment of a system with intelligent guidance and inspection function to provide intelligent guidance for patients. Method/Process Taking patients of the department of laboratory medicine as the investigation objects, questionnaires are used to obtain and analyze the difficulties and pain points of patients. The use case diagram, time sequence diagram and deployment diagram of the system are designed by using Enterprise Architect software and unified modeling language (UML), and the knowledge base of the system is designed by taking the determination of urinary microprotein creatinine ratio as an example. The system is optimized according to the results of evaluation questionnaires. Result/Conclusion The design method of an intelligent guidance and inspection system based on UML is feasible, and the system has been widely recognized, which provides a new idea for promoting structured, intelligent and knowledgeable electronic medical record (EMR) and improving medical quality.