Purpose/Significance With regional collaboration as the core, the study explores an efficient, convenient and standardized two-way referral service mode, further implements the hierarchical diagnosis and treatment system, and realizes the rational allocation of medical resources. Method/Process Combined with the “internet+”, a two-way referral cloud platform is built to realize the interconnection and information sharing between the referral business and the hospital business. Result/Conclusion The construction of the two-way referral cloud platform has promoted the standardization and convenience of referral process and improved the satisfaction of doctors and patients. The two-way referral service mode based on regional collaboration promotes the integration of medical resources, improves the medical service capacity of medical alliances, and improves the medical experience of referral patients.