Purpose/Significance To review the knowledge discovery methods based on knowledge graph in the biomedical field, and to provide references for researchers.Methods/Process The paper summarizes the knowledge discovery methods based on knowledge graph by systematically searching and analyzing the relevant literatures,and compares the advantages and disadvantages of various knowledge discovery methods. It points out that the limitations and challenges of knowledge discovery methods based on knowledge graph in the biomedical field, and puts forward suggestions and prospects.Result/Conclusion In the future research, it is suggested to improve the interpretability of knowledge discovery results, build an effective result evaluation framework, and establish a standardized knowledge discovery process with multi-domain experts’ collaboration, so as to improve the quality and efficiency of knowledge discovery research.