Significance To identify the influencing factors of users’ willingness to use knowledge service of hospital library. Method/Process Based on the technology acceptance model, the theory of expectation confirmation model and perceived risk theory, the model and hypothesis of users’ willingness to continue using knowledge service of hospital library are established. A questionnaire is designed and made, which is distributed to the staffs of Affiliated Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology by random sampling method. The structural equation model is used to validate and analyze the conceptual model.Result/Conclusion The findings show that expectation confirmation, perceived usefulness, perceived ease of use, and perceived interactivity all have positive effects on satisfaction. Of these, perceived usefulness is the most significant factor. Therefore, the key to ensure that users continue to use the library knowledge service is to enhance the usefulness of the service.