Purpose/Significance Aiming at biomedical engineering majors in medical colleges, the paper explores the effective path of first-class curriculum construction, cultivates innovative biomedical engineering talents, and improves students’ problem-solving ability. Method/Process Taking MATLAB program design and application course as an example, the study adopts the “high-level, innovative and challenging” standards to optimize the curriculum content, expand teaching methodologies, and update instructional models. Based on the advantages of the subject, the reform and application of teaching material construction and evaluation are carried out. Result/Conclusion The implemented reforms significantly enhance the students’ autonomous learning capabilities and learning effects, promote teachers’ teaching level, and provide useful references for the construction of similar courses in medical colleges.