Purpose/Significance To analyze the dynamic changes, influencing factors and weak links of e-health literacy of middle-aged and elderly people, so as to provide references for improving e-health literacy of middle-aged and elderly people.Method/Process A questionnaire survey is conducted among 505 middle-aged and elderly people in Guangzhou, and the results are analyzed by using SPSS 24.0.Result/Conclusion The average score of e-health literacy of middle-aged and elderly people is in the middle level; The weak link of e-health literacy is the lack of health information interaction and evaluation capacity. Individual factors are the primary factors that affect e-health literacy of middle-aged and elderly people. It is suggested to increase policy and financial support, carry out “the younger population helps the aging population” mode, and elderly-oriented transformation of digital health Apps.