Purpose/Significance To explore the teaching mode of Python language programming based on case-driven in medical universities and colleges, so as to cultivate the computational thinking ability of medical students and the ability to solve practical medical issues using computer programming ideas. Method/Process Detailed analysis is finished on the problems existing in the implementation of Python language programming course in medical universities and colleges. The content is innovated and the new teaching method based on cases is proposed. Taking the development of a text mining information system as an example, the process of teaching are elaborated in detail. Result/Conclusion The restructuring of the content and the reform of teaching mode improve the computational thinking ability of medical students and promote their ability to use computer technology to analyze and solve medical related issues.