Purpose/Significance From the perspective of practical teaching, the paper discusses the training methods of top talents in the field of bioinformatics, in order to realize the integration of theoretical knowledge and practical teaching, and cultivate compound and innovative talents.Methods/Process A teaching mode of “case-based teaching, scientific inquiry, and project-driven approach” is proposed and implemented. That mode guides students to pay attention to the application and transformation of theoretical knowledge with practical cases, promotes the intrinsic relevance of students’ learning with cutting-edge scientific issues, and encourages students to explore independently and stimulates their interest in learning with the project-driven teaching methods.Results/Conclusion The teaching mode can effectively improve students’ ability of self-analysis, self-reflection and open thinking, cultivate students' ability of innovation and practice, and provide an effective way for training high-quality talents in the field of bioinformatics.