Purpose/Significance To investigate medical students’ digital literacy level, and to propose cultivation strategies, so as to provide references for medical universities and colleges to carry out digital literacy education better.Method/Process Through a questionnaire survey, the digital literacy level of 907 medical undergraduates is investigated from 3 dimensions:medical knowledge acquisition and analysis, online learning and security, communication and collaboration.Result/Conclusion The overall level of digital literacy of medical students is high, especially in the use of digital tools for communication and collaboration, but the ability in medical knowledge acquisition and analysis is relatively poor. The digital literacy level of medical students in different grades and majors is different.In order to further improve the digital literacy level of medical students, we should pay attention to creating a personalized digital literacy education mode, constructing a practical digital literacy evaluation system, highlighting the characteristics of digital literacy in the medical field, and strengthening practical education on digital security.