Purpose/Significance To analyze the current status of cooperation between preprint systems and scientific journals at home and abroad, and to explore the collaborative development path of preprint systems and scientific journals in the biomedical field. Method/Process Through methods such as case analysis, online research, and literature review, 4 cooperation models between preprint systems and scientific journals are summarized. Combined with the construction practice of biomedRxiv, its cooperation mechanism with domestic and foreign journals is explored. Result/Conclusion biomedRxiv has formed collaborative models such as collaborative review, recommendation of high-quality manuscripts, and data sharing and referencing with the Taylor & Francis, Journal of Medical Informatics, etc, promoting the innovation of open academic exchange system in biomedicine in China. In the future, all parties need to work together to promote the deep integration of the preprint systems and scientific journals.