Purpose/Significance To fully leverage the value of eye movement physiological characteristics data, and to enrich the application of human physiological characteristics data, so as to provide reliable physiological data for disease diagnosis, and promote the development of medical and health care. Method/Process By using bibliometrics and literature review methods, the relevant literatures in CNKI and Web of Science databases are retrieved to summarize the current status of eye movement physiological characteristic research.Result/Conclusion It analyzes the research status of eye movement physiological characteristics from five aspects:the theory of eye movement physiological characteristics, common indicators, recording methods, processing methods and main application fields, and points out its shortcomings.