Purpose/Significance To construct a talent cultivation model for “AI+Medicine” micro-major, and to explore its optimization paths. Method/Process Taking 18 micro-major training programs of “AI+Medicine” in domestic universities as samples, the reflective thematic analysis method is adopted to conduct hierarchical coding and theme extraction of the programs, and the study is carried out in combination with the knowledge-ability-skill (KAS) theory and Dewey’s “New Three Centers” educational thought.Result/Conclusion The KAS-E framework is proposed, through which two aspects, four dimensions, 13 core themes and 44 sub-themes are identified, revealing a diversified cultivation model based on the synergy of knowledge, ability, skill and evaluation. Accordingly, a talent cultivation model for the “AI+Medicine” micro-major is constructed, five optimization paths are summarized, and the internal logic of talent cultivation in this field is clarified.